Article
Biochemical basis of hyper-recombinogenic activity of Pseudomonas aeruginosa RecA protein in Escherichia coli cells.
Molecular microbiology - 1 Feb 1998
Namsaraev E A, Baitin D, Bakhlanova I V, Alexseyev A A, Ogawa H, Lanzov V A
Abstract excerpt
The replacement of Escherichia coli recA gene (recA[Ec]) with the Pseudomonas aeruginosa recA(Pa) gene in Escherichia coli cells results in constitutive hyper-recombination (high frequency of recombination exchanges per unit length of DNA) in the absence of constitutive SOS response. To understand the biochemical basis of this unusual in vivo phenotype, we compared in vitro the recombination properties of...
Topics
- Bacterial Proteins
- DNA
- DNA, Single-Stranded
- DNA-Binding Proteins
- Escherichia coli
- Phenotype
- Pseudomonas aeruginosa
- Rec A Recombinases
- Recombination, Genetic
- SOS Response, Genetics
- Serine Endopeptidases