Article
Regulation of the DNA methyltransferase by the Ras-AP-1 signaling pathway.
The Journal of biological chemistry - 27 Jan 1995
Rouleau J, MacLeod A R, Szyf M
Abstract excerpt
Using deletion analysis and site-specific mutagenesis to map the 5' regulatory region of the DNA methyltransferase (MeTase) gene, we show that a 106-bp sequence (at -1744 to -1650) bearing three AP-1 sites is responsible for induction of DNA MeTase promoter activity. Using transient cotransfection chloramphenicol acetyl-transferase assays in P19 cells, we show that the DNA MeTase promoter is induced by c-Jun or...
Topics
- Animals
- Base Sequence
- Binding Sites
- Cell Line
- Cell Nucleus
- Chloramphenicol O-Acetyltransferase
- DNA Primers
- Deoxyribonuclease I
- Genes, ras
- Methyltransferases
- Mice
- Molecular Sequence Data