Article
Analysis of the mouse Dhfr/Rep-3 major promoter region by using linker-scanning and internal deletion mutations and DNase I footprinting.
Molecular and cellular biology - 1 Nov 1990
Smith M L, Mitchell P J, Crouse G F
Abstract excerpt
The mouse dihydrofolate reductase (Dhfr) promoter region is buried within a CpG island (a region rich in unmethylated CpG dinucleotides), has a high G+C content, and lacks CAAT and TATA elements. The region contains four 48-bp repeats, each of which contains an Sp1-binding site. Another gene, Rep...
Topics
- Animals
- Base Sequence
- Chloramphenicol O-Acetyltransferase
- Chromosome Deletion
- Deoxyribonuclease I
- Genes
- Mice
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Tetrahydrofolate Dehydrogenase
- Transcription, Genetic
- Transfection