Article
Mutation, loss of heterozygosity, and recombination of the p53 gene in mouse forestomach tumors induced by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline.
Molecular carcinogenesis - 1 Jan 1995
Ushijima T, Makino H, Okonogi H, Hosoya Y, Sugimura T, Nagao M
Abstract excerpt
2-Amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), a food mutagen, induces forestomach tumors in CDF1 mice. We established a polymerase chain reaction (PCR)-single-strand conformation polymorphism (SSCP) analysis system to detect mutations in the mouse p53 gene exons 2-10, which encompass all fi...
Topics
- Animals
- Base Sequence
- Carcinogens
- Chromosome Deletion
- Genes, p53
- Heterozygote
- Mice
- Mice, Inbred BALB C
- Mice, Inbred DBA
- Molecular Sequence Data
- Mutagens
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Quinolines
- Recombination, Genetic
- Stomach Neoplasms
- Tumor Cells, Cultured