Identification of two mismatch-binding activities in protein extracts of Schizosaccharomyces pombe.

doi:10.1093/nar/22.24.5289

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Article

Identification of two mismatch-binding activities in protein extracts of Schizosaccharomyces pombe.

Nucleic acids research - 11 Dec 1994

Fleck O, Schär P, Kohli J

Abstract excerpt

We have performed band-shift assays to identify mismatch-binding proteins in cell extracts of Schizosaccharomyces pombe. By testing heteroduplex DNA containing either a T/G or a C/C mismatch, two distinct band shifts were produced in the gels. A low mobility complex was observed with the T/G subs...

Topics

Base Sequence
DNA, Fungal
DNA, Single-Stranded
DNA-Binding Proteins
Fungal Proteins
Molecular Sequence Data
Mutation
Nucleic Acid Heteroduplexes
Polydeoxyribonucleotides
Saccharomyces cerevisiae Proteins
Schizosaccharomyces
Transcription Factors

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