Article
Riboflavin biosynthesis in Saccharomyces cerevisiae. Cloning, characterization, and expression of the RIB5 gene encoding riboflavin synthase.
The Journal of biological chemistry - 6 Jan 1995
Santos M A, García-Ramírez J J, Revuelta J L
Abstract excerpt
Saccharomyces cerevisiae has a monofunctional riboflavin synthase that catalyzes the formation of riboflavin from 6,7-dimethyl-8-ribityllumazine. We have isolated the gene encoding this enzyme from a yeast genomic library by functional complementation of a mutant, rib5-10, lacking riboflavin synt...
Topics
- Base Sequence
- Cloning, Molecular
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Protein Conformation
- RNA, Messenger
- Riboflavin
- Riboflavin Synthase
- Saccharomyces cerevisiae
