Article
The proline-dependent transcription factor Put3 regulates the expression of the riboflavin transporter MCH5 in Saccharomyces cerevisiae.
Genetics - 1 Dec 2008
Spitzner Andrea, Perzlmaier Angelika F, Geillinger Kerstin E, Reihl Petra, Stolz Jürgen
Abstract excerpt
Like most microorganisms, the yeast Saccharomyces cerevisiae is prototrophic for riboflavin (vitamin B2). Riboflavin auxotrophic mutants with deletions in any of the RIB genes frequently segregate colonies with improved growth. We demonstrate by reporter assays and Western blots that these suppressor mutants overexpress the plasma-membrane riboflavin transporter MCH5. Frequently, this overexpression is mediated...
Topics
- Binding Sites
- Gene Expression Regulation, Fungal
- Genetic Complementation Test
- Models, Biological
- Monocarboxylic Acid Transporters
- Phenotype
- Proline
- Promoter Regions, Genetic
- Riboflavin
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Suppression, Genetic