Article
Rapid identification of specific mutations in the sequence of an enzyme variant produced by protein engineering using high-performance liquid chromatographic/fast atom bombardment mass spectrometric techniques.
Biological mass spectrometry - 1 Nov 1994
van Dongen W D, van Bommel J H, van Wassenaar P D, Heerma W, Haverkamp J
Abstract excerpt
Unknown, specific mutations in the sequence of an enzyme variant (a Bacillus subtilisin protease) produced by protein engineering were identified using High-performance Liquid Chromatographic/Fast Atom Bombardment Mass Spectrometric (HPLC/FAB MS) techniques. The variant and the highly homologous wild-type enzyme were treated with CNBr followed by tryptic digestion. The resulting peptides were analysed using...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Chromatography, High Pressure Liquid
- Cyanogen Bromide
- Genetic Engineering
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Recombinant Proteins