Article
Detection of blood chimerism after lung and heart-lung transplantation. The superiority of nested as compared with standard polymerase chain reaction amplification.
Transplantation - 27 Dec 1994
Knoop C, Andrien M, Defleur V, Antonine M, de Francquen P, Goldman M, Estenne M
Abstract excerpt
Migration of donor cells from the graft to various tissues of the recipient has been demonstrated after different types of solid organ transplants. Currently, the detection of donor cells in the recipient's tissues is most simply performed by polymerase chain reaction (PCR) amplification of a donor-specific gene. In the present study, we first determined in vitro the sensitivity of standard and nested PCR...
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