Article
Reliable quantification of hematopoietic chimerism after allogeneic transplantation for acute leukemia using amplification by real-time PCR of null alleles and insertion/deletion polymorphisms.
Leukemia - 1 Mar 2005
Jiménez-Velasco A, Barrios M, Román-Gómez J, Navarro G, Buño I, Castillejo J A, Rodríguez A I, García-Gemar G, Torres A, Heiniger A I
Abstract excerpt
Increasing mixed chimerism (MC) after allogeneic stem cell transplantation (SCT) has been associated with a high risk of relapse in acute leukemia. We evaluated a new method for chimerism detection, based on the quantitative real-time PCR (qrt-PCR) amplification of null alleles or insertion/deletion polymorphisms (indels). All qrt-PCR assays with null alleles and indels attained a sensitivity of at least 10(-4),...
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