Article
Approaching the multifaceted nature of energy metabolism: inactivation of the cytosolic creatine kinases via homologous recombination in mouse embryonic stem cells.
Molecular and cellular biochemistry - 1 Jan 1994
van Deursen J, Wieringa B
Abstract excerpt
To study the physiological role of the creatine kinase/phosphocreatine (CK/PCr) system in cells and tissues with a high and fluctuating energy demand we have concentrated on the site-directed inactivation of the B- and M-CK genes encoding the cytosolic CK protein subunits. In our approach we used homologous recombination in mouse embryonic stem (ES) cells from strain 129/Sv. Using targeting constructs based on...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.