Nucleoside 2-deoxyribosyltransferase. Pre-steady-state kinetic analysis of native enzyme and mutant enzyme with an alanyl residue replacing Glu-98.

doi:10.1074/jbc.270.26.15557

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Article

Nucleoside 2-deoxyribosyltransferase. Pre-steady-state kinetic analysis of native enzyme and mutant enzyme with an alanyl residue replacing Glu-98.

The Journal of biological chemistry - 30 Jun 1995

Porter D J, Short S A

Abstract excerpt

Nucleoside 2-deoxyribosyltransferase catalyzes cleavage of a 2'-deoxyribosylnucleoside (A) to a nucleobase (P) with deoxyribosylation of the enzyme. Substrates quenched the intrinsic fluorescence of native enzyme (E) and a catalytically inactive mutant enzyme (E98A enzyme). The time courses of th...

Topics

Adenine
Alanine
Deoxyadenosines
Deoxyuridine
Fluorescence
Glutamic Acid
Kinetics
Mutation
Pentosyltransferases
Structure-Activity Relationship
Thymidine

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