Article
Evidence against either a premature stop codon or the absence of obese gene mRNA in human obesity.
The Journal of clinical investigation - 1 Jun 1995
Considine R V, Considine E L, Williams C J, Nyce M R, Magosin S A, Bauer T L, Rosato E L, Colberg J, Caro J F
Abstract excerpt
Obese (ob) gene expression in abdominal subcutaneous adipocytes from lean and obese humans was examined. The full coding region of the ob gene was isolated from a human adipocyte cDNA library. Translation of the insert confirmed the reported amino acid sequence. There was no difference in the sequence of an reverse transcription PCR product of the coding region from five lean and five obese subjects. The nonsense...
Topics
- Adult
- Amino Acid Sequence
- Animals
- Base Sequence
- Body Mass Index
- Cloning, Molecular
- DNA Primers
- DNA, Complementary
- Female
- Gene Expression
- Genes