Article
TmrB protein, which confers resistance to tunicamycin on Bacillus subtilis, binds tunicamycin.
Bioscience, biotechnology, and biochemistry - 1 Feb 1995
Noda Y, Takatsuki A, Yoda K, Yamasaki M
Abstract excerpt
Overproduction of TmrB protein, a 22.5-kDa protein with an N-terminal ATP-binding region and a C-terminal amphiphilic alpha-helix, confers resistance to tunicamycin on Bacillus subtilis. TmrB protein was found to bind Sepharose 6B to which tunicamycin was covalently linked. Experiments with mutan...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Binding Sites
- Binding, Competitive
- Blotting, Western
- Chemical Fractionation
- Drug Resistance, Microbial
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Bacterial
- Membrane Proteins
- Molecular Weight
- Mutation
- Precipitin Tests
- Tunicamycin