Article
Deletion of a single helix from the transmembrane domain causes large changes in membrane insertion properties and secondary structure of the bacterial conjugation protein TrwB.
Biochimica et biophysica acta - 1 Dec 2012
Vecino Ana Julia, Segura Rosa de Lima, de la Arada Igor, de la Cruz Fernando, Goñi Félix M, Arrondo José L, Alkorta Itziar
Abstract excerpt
TrwB is an essential protein in the conjugative transfer of plasmid R388. The protein consists of a bulky cytosolic domain containing the catalytic site, and a small transmembrane domain (TMD). Our previous studies support the idea that the TMD plays an essential role in the activity, structure and stability of the protein. We have prepared a mutant, TrwBΔN50 that lacks one of the two α-helices in the TMD. The...
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