Article
Pyruvic acid production by an F1-ATPase-defective mutant of Escherichia coli W1485lip2.
Bioscience, biotechnology, and biochemistry - 1 Dec 1994
Yokota A, Terasawa Y, Takaoka N, Shimizu H, Tomita F
Abstract excerpt
An F1-ATPase-defective mutant, TBLA-1, was constructed by the transduction of a defective gene for the alpha subunit of F1-ATPase, atpA401, into Escherichia coli W1485lip2, a lipoic acid-requiring pyruvic acid producer. The pyruvic acid production of the strain TBLA-1 was found to be improved markedly compared with that of strain W1485lip2. In cultures using a jar fermentor, the strain W1485lip2 consumed 50...
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