Article
Characterization of semi-uncoupled hybrid Escherichia coli-Bacillus megaterium F1F0 proton-translocating ATPases.
The Journal of biological chemistry - 5 Oct 1991
Scarpetta M A, Hawthorne C A, Brusilow W S
Abstract excerpt
Cloned atp genes for the proton-translocating ATPase of the obligate aerobe Bacillus megaterium have been demonstrated to be capable of complementing Escherichia coli ATPase (unc) mutants (Hawthorne, C. A., and Brusilow, W. S. A. (1986) J. Biol. Chem. 261, 5245-5248). To determine the minimum subunit requirements for cross-species complementation, we constructed all combinations of B. megaterium atpA, G, D, and C...
Topics
- Bacillus megaterium
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genetic Complementation Test
- Immunoblotting
- Molecular Sequence Data
- Mutation
- Plasmids
- Proton-Translocating ATPases
