Article
Heterologous gene expression in continuous cultures of budding yeast.
Applied microbiology and biotechnology - 1 Feb 1991
Porro D, Martegani E, Ranzi B M, Alberghina L
Abstract excerpt
In a previous paper we have studied the expression of beta-galactosidase from Escherichia coli, driven from the inducible GAL1-10/CYC1 hybrid promoter, in batch cultures of budding Saccharomyces cerevisiae and have described operating conditions for maximal productivity. In this paper we show that the plasmid instability in continuous cultures can be overcome by utilizing appropriate selection markers and a high...
Topics
- Escherichia coli
- Gene Amplification
- Gene Expression
- Genetic Markers
- Genetic Variation
- Oxygen
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae
- beta-Galactosidase