Article
Use of polymerase chain reaction (PCR) in the development of a recombinant organism.
Developments in biological standardization - 1 Jan 1994
Helder J C, Utter S L
Abstract excerpt
Tryptic mapping of a purified recombinant antibody expressed in a mammalian cell line demonstrated low level heterogeneity in the heavy chain. Amino acid analysis and N-terminal sequencing of these fragments revealed that a conversion of tyrosine (Y) to glutamine (Q) had occurred at residue 376....
Topics
- Animals
- Antibodies, Monoclonal
- Base Sequence
- Biotechnology
- CHO Cells
- Cloning, Molecular
- Cricetinae
- DNA Primers
- DNA, Recombinant
- Escherichia coli
- Genetic Variation
- Humans
- Molecular Sequence Data
- Peptide Mapping
- Plasmids
- Polymerase Chain Reaction
- Quality Control
- Recombinant Proteins