Use of polymerase chain reaction (PCR) in the development of a recombinant organism.

Abstract excerpt

Tryptic mapping of a purified recombinant antibody expressed in a mammalian cell line demonstrated low level heterogeneity in the heavy chain. Amino acid analysis and N-terminal sequencing of these fragments revealed that a conversion of tyrosine (Y) to glutamine (Q) had occurred at residue 376. DNA sequencing of 30 clones of the original expression construct indicated that the chance of the Y376Q variant being...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Base Sequence
• Share to Cloning, Molecular
• Share to DNA Primers