Identifying bottlenecks in transient and stable production of recombinant monoclonal‐antibody sequence variants in chinese hamster ovary cells

Abstract excerpt

The increasing demand for antibody-based therapeutics has emphasized the need for technologies to improve recombinant antibody titers from mammalian cell lines. Moreover, as antibody therapeutics address an increasing spectrum of indications, interest has increased in antibody engineering to improve affinity and biological activity. However, the cellular mechanisms that dictate expression and the relationships...