Article
Involvement of the cysteine-rich domain of glycoprotein IIIa in the expression of the human platelet alloantigen, PlA1: evidence for heterogeneity in the humoral response.
Blood - 1 Jun 1995
Valentin N, Visentin G P, Newman P J
Abstract excerpt
To assess the potential contribution of the cysteine-rich domain of human platelet glycoprotein (GP) IIIa to the structure of the PlA1 epitope, we used site-directed mutagenesis to substitute alanines for cysteines at key positions potentially involved in PlA1 alloantigen formation. One of these GPIIIa isoforms in which alanine replaced Cys435 reacted normally with a series of well-characterized murine MoAbs...
Topics
- Alleles
- Antibodies, Monoclonal
- Antigens, Human Platelet
- Base Sequence
- Cysteine
- Humans
- Integrin beta3
- Isoantibodies
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Platelet Membrane Glycoproteins