Article
The catalytic mechanism of Fpg protein. Evidence for a Schiff base intermediate and amino terminus localization of the catalytic site.
The Journal of biological chemistry - 12 May 1995
Tchou J, Grollman A P
Abstract excerpt
Our recent structure-activity analysis of Fpg protein of Escherichia coli, using oligodeoxynucleotides containing various 8-oxopurine derivatives, has allowed us to postulate an enzyme mechanism involving protonation of 8-oxoguanine at O-6 and nucleophilic attack of the deoxyribose moiety at C-1'...
Topics
- ATP-Binding Cassette Transporters
- Base Sequence
- Binding Sites
- Carrier Proteins
- DNA-Formamidopyrimidine Glycosylase
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Escherichia coli Proteins
- Factor Xa
- Genes, Bacterial
- Genotype
- Maltose-Binding Proteins
- Molecular Sequence Data
- Monosaccharide Transport Proteins
- Mutagenesis, Site-Directed
- N-Glycosyl Hydrolases
- Oligodeoxyribonucleotides
- Peptide Fragments