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Article

The catalytic mechanism of Fpg protein. Evidence for a Schiff base intermediate and amino terminus localization of the catalytic site.

The Journal of biological chemistry - 12 May 1995

Tchou J, Grollman A P

Abstract excerpt

Our recent structure-activity analysis of Fpg protein of Escherichia coli, using oligodeoxynucleotides containing various 8-oxopurine derivatives, has allowed us to postulate an enzyme mechanism involving protonation of 8-oxoguanine at O-6 and nucleophilic attack of the deoxyribose moiety at C-1' leading to the formation of an enzyme-substrate Schiff base intermediate (Tchou, J., Bodepudi, V., Shibutani, S.,...

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Identifiers

PMID: 7744806
DOI: 10.1074/jbc.270.19.11671

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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