Article
Isoelectric focusing in immobilized pH gradient of melon (Cucumis melo L.) seed protein: methodical and genetic aspects, and application in breeding.
Electrophoresis - 1 Dec 1994
van den Berg B M, Gabillard D
Abstract excerpt
Genetic variability of melon seed proteins was studied by separation of seed protein by isoelectric focusing in immobilized pH gradient (IEF-IPG) under denaturing conditions. A routine procedure was developed for IEF-IPG of hundreds of individual melon seeds per day. A group of 74 accessions from 19 morphologically distinct groups and from different geographic origin were studied by IEF-IPG using pH gradients of...
Topics
- Breeding
- Fruit
- Genetic Variation
- Hydrogen-Ion Concentration
- Isoelectric Focusing
- Phylogeny
- Plant Proteins
- Seeds
