Article
Variety identification in maize lines via capillary electrophoresis of zeins in isoelectric acidic buffers.
Electrophoresis - 1 Jun 1999
Olivieri E, Viotti A, Lauria M, Simò-Alfonso E, Righetti P G
Abstract excerpt
Zeins (the prolamins or seed storage proteins in maize) have been used to characterize and identify different genotypes. Zeins were fractionated by capillary zone electrophoresis in acidic, amphoteric buffers, which represent a medium of moderate conductivity and are thus compatible with higher voltage gradients. The running buffer consisted of 40 mM isoelectric aspartic acid, in presence of 6 M urea and 0.5%...
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