Article
HLA typing for DR3 and DR4 using artificial restriction fragment length polymorphism PCR from archival DNA.
Journal of clinical pathology - 1 Jan 1995
Horton V A, Bunce M, Davies D R, Turner R C, Lo Y M
Abstract excerpt
AIM: To develop polymerase chain reaction based artificial restriction fragment length polymorphism (artificial RFLP PCR) assays for DR3 and DR4 alleles of the multiallelic DRB1 locus and to apply them to paraffin wax embedded archival material. METHODS: Sixty five samples from DRB1 typed cell lines were analysed using the artificial RFLP PCR method to determine the specificity and sensitivity of the system....
Topics
- Alleles
- Base Sequence
- Cell Line
- HLA-DR3 Antigen
- HLA-DR4 Antigen
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sensitivity and Specificity