Article
HLA DQ alpha typing of forensic specimens by amplification restriction fragment polymorphism (ARFP) analysis.
Forensic science international - 1 Oct 1991
Harrington C S, Dunaiski V, Williams K E, Fowler C
Abstract excerpt
The alleles present at the HLA DQ alpha locus may be typed by either allele specific oligonucleotide (ASO) probing or by restriction mapping, referred to here as amplification restriction fragment polymorphism (ARFP) analysis. ASO typing relies upon hybridization principles, whereas ARFP typing relies upon restriction site analysis. Dot-blot ASO typings which are of doubtful interpretation may be directly checked...
Topics
- Alleles
- DNA
- Electrophoresis, Agar Gel
- Forensic Medicine
- Gene Frequency
- Genotype
- HLA-DQ Antigens
- Humans
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length