Article
Functional consequences in yeast of single-residue alterations in a consensus calmodulin.
Journal of cell science - 1 Nov 1994
Harris E, Watterson D M, Thorner J
Abstract excerpt
A synthetic gene encoding a 'consensus' calmodulin (synCaM) was able to substitute for the Saccharomyces cerevisiae calmodulin gene (CMDI), even though synCaM is only 60% identical in primary amino acid sequence to yeast Cmd1. Twelve different synCaM mutants were also expressed in yeast. Seven of the 12 mutant synCaMs supported germination and growth of Cmd1-deficient spores. Five of the 12 mutant synCaMs were...
Topics
- Calmodulin
- Consensus Sequence
- Electrochemistry
- Gene Expression
- Genes, Fungal
- Microtubules
- Mitosis
- Models, Molecular
- Mutagenesis, Site-Directed
- Phenotype
- Ploidies
- Protein Structure, Secondary