Article
Mutations which block the binding of calmodulin to Spc110p cause multiple mitotic defects.
Journal of cell science - 1 Jun 1996
Stirling D A, Rayner T F, Prescott A R, Stark M J
Abstract excerpt
We have generated three temperature-sensitive alleles of SPC110, which encodes the 110 kDa component of the yeast spindle pole body (SPB). Each of these alleles carries point mutations within the calmodulin (CaM) binding site of Spc110p which affect CaM binding in vitro; two of the mutant protein...
Topics
- Alleles
- Binding Sites
- Calmodulin
- Calmodulin-Binding Proteins
- Cytoskeletal Proteins
- DNA Replication
- Fungal Proteins
- Immunohistochemistry
- Microtubules
- Mitosis
- Nuclear Proteins
- Point Mutation
- Protein Binding
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Spindle Apparatus
- Temperature