Isolation of Escherichia coli mutants lacking methylcytosine-dependent restriction systems for cloning extensively methylated frog virus 3 DNA.

doi:10.1016/0378-1119(93)90673-q

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Article

Isolation of Escherichia coli mutants lacking methylcytosine-dependent restriction systems for cloning extensively methylated frog virus 3 DNA.

Gene - 6 Sept 1993

Dy L, Chalasani S, Essani K

Abstract excerpt

Many bacterial strains possess methylation-dependent restriction systems (MDRS) that demonstrate methylcytosine-dependent restriction endonuclease activity for the dinucleotide sequence, dCpdG. This makes these strains unsuitable for cloning methylated DNA. Some commercially available bacterial cells are recommended for cloning DNA fragments with methylated cytosines and adenines, e.g., Escherichia coli DH5-alpha...

Topics

5-Methylcytosine
Ampicillin Resistance
Azacitidine
Base Sequence
Clone Cells
Cloning, Molecular
Cytosine
DNA Transposable Elements
DNA, Viral
DNA-Binding Proteins
DNA-Cytosine Methylases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Electrophoresis, Polyacrylamide Gel

Escherichia coli

Iridoviridae

Methylation

Molecular Sequence Data

Mutation

Plasmids

Source

Open in PubMed

Identifiers

PMID: 7690339
DOI: 10.1016/0378-1119(93)90673-q

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7690339-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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