Article
Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system.
Virology - 1 Jul 1991
Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y
Abstract excerpt
Full-length DNA copies of RNAs 1, 2, and 3 of CMV Y strain (CMV-Y) were cloned downstream of modified phage T7 promoter sequences to obtain infectious RNA transcripts. The small number of extra nonviral nucleotides at the 5' ends considerably decreased the specific infectivity of the transcripts of RNAs 1 and 2 but did not affect that of the RNA3 transcripts. Using the most infective transcripts, up to 45% of...
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