Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system.

doi:10.1016/0042-6822(91)90123-s

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Article

Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system.

Virology - 1 Jul 1991

Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y

Abstract excerpt

Full-length DNA copies of RNAs 1, 2, and 3 of CMV Y strain (CMV-Y) were cloned downstream of modified phage T7 promoter sequences to obtain infectious RNA transcripts. The small number of extra nonviral nucleotides at the 5' ends considerably decreased the specific infectivity of the transcripts...

Topics

Base Sequence
Capsid
Chromosome Deletion
DNA, Viral
Molecular Sequence Data
Mosaic Viruses
Mutation
Plants, Toxic
Protoplasts
RNA, Viral
Nicotiana
Transcription, Genetic

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