Article
Dissecting the cellular functions of annexin XI using recombinant human annexin XI-specific autoantibodies cloned by phage display.
The Journal of biological chemistry - 29 Aug 2003
Farnaes Lauge, Ditzel Henrik J
Abstract excerpt
Functional studies of cellular proteins are often complicated by the lack of well-defined monoclonal antibodies, the production of which is hampered by the highly conserved nature of these cellular proteins across species. Annexin XI, a member of the Ca2+-dependent, phospholipid-binding protein family, is an example of such a protein and was used in studies to devise a strategy using human autoimmune phage...
Topics
- Amino Acid Sequence
- Animals
- Annexins
- Antibodies, Monoclonal
- Autoantibodies
- Blotting, Western
- COS Cells
- Calcium
- Cell Line
- Cell Nucleus
- Cells, Cultured
- Cloning, Molecular
- Cytoplasm
- DNA, Complementary