Article
Co-overexpression of RspAB improves recombinant protein production in Escherichia coli.
Metabolic engineering - 1 Oct 2000
Weikert C, Canonaco F, Sauer U, Bailey J E
Abstract excerpt
The Escherichia coli mutant CWML2 was previously reported to exhibit improved physiological characteristics, including recombinant protein production. Here we investigate the molecular basis of this phenotype by comparing the cellular level of three RNA polymerase sigma subunits by immunoblot analysis. While the level of housekeeping sigma(D) was similar in parent and mutant, the levels of the flagella synthesis...
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