Article
Segregation of mitochondrial DNAs carrying a pathogenic point mutation (tRNA(leu3243)) in cybrid cells.
Biochemical and biophysical research communications - 4 Aug 1995
Shoubridge E A
Abstract excerpt
To investigate the mechanism of segregation of mitochondrial DNAs (mtDNAs) carrying a pathogenic point mutation in the tRNA(leu(UUR)) gene (A to G at position 3243) cytoplasts, derived from a heteroplasmic myoblast clone, were fused to rhoo cells to create cybrid cells carrying different proportions of mutant and wild-type mtDNAs. Although the individual myoblasts used as fusion partners contained predominantly...
Topics
- Adenine
- Animals
- Bone Neoplasms
- Cell Fusion
- Clone Cells
- DNA, Mitochondrial
- Genotype
- Guanine
- Humans
- Hybrid Cells
- Muscles
- Osteosarcoma