Article
Genotypic stability, segregation and selection in heteroplasmic human cell lines containing np 3243 mutant mtDNA.
Genetics - 1 Jan 2000
Lehtinen S K, Hance N, El Meziane A, Juhola M K, Juhola K M, Karhu R, Spelbrink J N, Holt I J, Jacobs H T
Abstract excerpt
The mitochondrial genotype of heteroplasmic human cell lines containing the pathological np 3243 mtDNA mutation, plus or minus its suppressor at np 12300, has been followed over long periods in culture. Cell lines containing various different proportions of mutant mtDNA remained generally at a consistent, average heteroplasmy value over at least 30 wk of culture in nonselective media and exhibited minimal mitotic...
Topics
- Base Sequence
- Cytoskeleton
- DNA Primers
- DNA, Mitochondrial
- Dimethyl Sulfoxide
- Genotype
- Humans
- Mutation
- Phenotype
- Selection, Genetic
- Tumor Cells, Cultured