Article
[p53 with a mutation in codon 273 increases the probability of amplifying the dhfr gene in Rat-1 and LIM1215 cells].
Genetika - 1 May 1995
Il'inskaia G V, Pugacheva E N, Sokova O I, Chumakov P M, Kopnin B P
Abstract excerpt
The effect of the expression of the exogenous human mutant p53 (Arg-->His in codon 273) on the amplification rate of the gene dhfr in permissive Rat-1 and LIM1215 cells was studied. It was shown that injection of a retroviral construction with p53His273 resulted in the accumulation of methotrexate-resistant variants with an increased number of dhfr copies in populations of recipient cells. Luria-Delbruck...
Topics
- Animals
- Cell Line
- Chromosomes
- Cloning, Molecular
- Codon
- Drug Resistance
- Gene Amplification
- Humans
- Methotrexate
- Mutation
- Rats
- Recombination, Genetic