Article
Specific, nonproductive cleavage of packaged bacteriophage T7 DNA in vivo.
Virology - 10 Jul 1995
Khan S A, Hayes S J, Watson R H, Serwer P
Abstract excerpt
The morphogenesis of bacteriophage T7 includes assembly of a procapsid that subsequently both packages DNA and changes in structure. The DNA packaged by T7 is concatemeric and is cleaved to mature length during packaging. In the present study, packaged DNA obtained from T7-infected cells was analyzed after release from DNase-treated capsids. After fractionation by agarose gel electrophoresis, in-gel probing with...
Topics
- Bacteriophage T7
- Capsid
- DNA, Viral
- Escherichia coli
- Morphogenesis
- Mutation
- Repetitive Sequences, Nucleic Acid
- Temperature
- Virus Replication
