Article
Role of gene 6 exonuclease in the replication and packaging of bacteriophage T7 DNA.
Journal of molecular biology - 20 Sept 1990
Serwer P, Watson R H, Son M
Abstract excerpt
When bacteriophage T7 gene 6 exonuclease is genetically removed from T7-infected cells, degradation of intracellular T7 DNA is observed. By use of rate zonal centrifugation, followed by either pulsed-field agarose gel electrophoresis or restriction endonuclease analysis, in the present study, the following observations were made. (1) Most degradation of intracellular DNA requires the presence of T7 gene 3...
Topics
- Centrifugation, Density Gradient
- DNA Replication
- DNA, Viral
- Electrophoresis, Agar Gel
- Exonucleases
- Genes, Viral
- Morphogenesis
- Mutation
- Restriction Mapping
- T-Phages
- Viral Proteins