Article
SSCP primer design based on single-strand DNA structure predicted by a DNA folding program.
Nucleic acids research - 25 Jun 1995
Nielsen D A, Novoradovsky A, Goldman D
Abstract excerpt
To predict alterations in single-strand DNA mobility in non-denaturing electrophoretic gels, Zuker's RNA folding program was modified. Energy files utilized by the LRNA RNA folding algorithm were modified to emulate folding of single-strand DNA. Energy files were modified to disallow G-T base pairing. Stacking energies were corrected for DNA thermodynamics. Constraints on loop nucleotide sequences were removed....
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