Biophysical characterization of double-stranded oligonucleotides using ETBR and isothermal fluorescence spectroscopy: implication for SNP genotyping.

doi:10.1016/j.jprot.2007.10.001

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Article

Biophysical characterization of double-stranded oligonucleotides using ETBR and isothermal fluorescence spectroscopy: implication for SNP genotyping.

Journal of biochemical and biophysical methods - 24 Apr 2008

Bhattacharya Pradip, Sharma Swarkar, Gochhait Sailesh, Bamezai Rameshwar N K

Abstract excerpt

The UV-absorption, fluorescence and CD spectra of aps 23 bp oligoduplexes were performed for potential diagnostic purpose. These oligonucleotide sequences were mimicked from natural mutations (mitochondrial genome) of human population (unpublished). This work was designed on the basis of hybridization of non-self complementary oligoduplexes (aps) containing no mismatch, one-mismatch and two-mismatches. Since...

Topics

Apoptosis Regulatory Proteins
BRCA2 Protein
Biophysical Phenomena
Biophysics
Circular Dichroism
Ethidium
Genotype
Humans
Oligonucleotides
Polymorphism, Single Nucleotide
Spectrometry, Fluorescence
Temperature
Thermodynamics

Source

Open in PubMed

Identifiers

PMID: 18177945
DOI: 10.1016/j.jprot.2007.10.001

Genes

BRCA2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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