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Article

Selection of mutations altering specificity in restriction-modification enzymes using the bacteriophage P22 challenge-phage system.

Gene - 19 May 1995

Fisher E W, Yang M T, Jeng S T, Gardner J F, Gumport R I

Abstract excerpt

A method for selecting mutants of site-specific DNA-binding proteins has been applied to the study of the EcoRI and RsrI restriction-modification enzymes. Catalytically inactive variants of both endonucleases are shown to function as pseudo-repressors in the bacteriophage P22 challenge-phage assay, and, upon further mutagenesis of the gene encoding R.EcoRI, a variant of that enzyme has been selected which appears...

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Identifiers

PMID: 7607471
DOI: 10.1016/0378-1119(95)00021-w

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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