Selection of mutations altering specificity in restriction-modification enzymes using the bacteriophage P22 challenge-phage system.

doi:10.1016/0378-1119(95)00021-w

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Article

Selection of mutations altering specificity in restriction-modification enzymes using the bacteriophage P22 challenge-phage system.

Gene - 19 May 1995

Fisher E W, Yang M T, Jeng S T, Gardner J F, Gumport R I

Abstract excerpt

A method for selecting mutants of site-specific DNA-binding proteins has been applied to the study of the EcoRI and RsrI restriction-modification enzymes. Catalytically inactive variants of both endonucleases are shown to function as pseudo-repressors in the bacteriophage P22 challenge-phage assa...

Topics

Bacteriophage P22
Base Sequence
Binding Sites
Catalysis
DNA-Binding Proteins
Genetic Variation
Mutagenesis, Site-Directed
Point Mutation
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Substrate Specificity

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