Article
Functional complementation of yeast phosphofructokinase mutants by the non-allosteric enzyme from Dictyostelium discoideum.
FEBS letters - 23 Oct 1995
Estévez A M, Heinisch J J, Aragón J J
Abstract excerpt
Phosphofructokinase (PFK) from yeast has been replaced by the non-allosteric isozyme from the slime mold Dictyostelium discoideum. This has been achieved by overexpression of the latter in a PFK-deficient strain of Saccharomyces cerevisiae under the control of the PFK2 promoter. Transformants com...
Topics
- Allosteric Regulation
- Animals
- Base Sequence
- DNA Primers
- Dictyostelium
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Phosphofructokinase-1
- Plasmids
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae