Article
Overexpression of wild-type and mutant NDP kinase in Dictyostelium discoideum.
Molecular microbiology - 1 Apr 1995
Sellam O, Véron M, Hildebrandt M
Abstract excerpt
Nucleoside diphosphate (NDP) kinase has a central role in the synthesis of (deoxy-)trinucleotides. In addition, mutations in the gene encoding NDP kinase have been shown to have important consequences for Drosophila development and mammalian tumorogenesis. We have overexpressed, in Dictyostelium discoideum, a genomic clone encoding the enzyme NDP kinase. The concomitant increase in the levels of RNA and enzyme...
Topics
- Animals
- Blotting, Northern
- Blotting, Western
- Dictyostelium
- Enzyme Stability
- Fungal Proteins
- Gene Expression Regulation, Developmental
- Mutation
- Nucleoside-Diphosphate Kinase
- Plasmids
- Protein Conformation