Article
Complementation of soluble phosphofructokinase activity in yeast mutants.
European journal of biochemistry - 15 Aug 1991
Gayatri A G, Maitra P K
Abstract excerpt
We describe here the genetic and biochemical analyses of two classes of mutations in the soluble phosphofructokinase (PFK I) of Saccharomyces cerevisiae: those leading to the loss of activity and those giving rise to a kinetically altered enzyme. Complementation and allele-testing between these two classes of mutants show that loss of enzyme activity in vitro can come about not only by mutations in the catalytic...
Topics
- Alleles
- Blotting, Western
- Centrifugation, Density Gradient
- Enzyme Activation
- Gene Expression Regulation, Enzymologic
- Kinetics
- Mutation
- Phenotype
- Phosphofructokinase-1
- Saccharomyces cerevisiae