Expression of heterologous phosphofructokinase genes in yeast.

doi:10.1016/0014-5793(93)80960-3

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Article

Expression of heterologous phosphofructokinase genes in yeast.

FEBS letters - 9 Aug 1993

Heinisch J J

Abstract excerpt

Genes encoding phosphofructokinases (PFK) from Escherichia coli and from the human muscle were expressed in PFK-deficient strains of Saccharomyces cerevisiae under the control of an inducible GAL1 promoter. They restored PFK activity under inducing conditions and complemented the galactose-negati...

Topics

Base Sequence
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Escherichia coli
Fructosediphosphates
Galactose
Gene Expression Regulation, Enzymologic
Humans
Microbiological Techniques
Molecular Sequence Data
Muscles
Phenotype
Phosphofructokinase-1
Plasmids
Saccharomyces cerevisiae

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