Article
Expression of heterologous phosphofructokinase genes in yeast.
FEBS letters - 9 Aug 1993
Heinisch J J
Abstract excerpt
Genes encoding phosphofructokinases (PFK) from Escherichia coli and from the human muscle were expressed in PFK-deficient strains of Saccharomyces cerevisiae under the control of an inducible GAL1 promoter. They restored PFK activity under inducing conditions and complemented the galactose-negative growth phenotype of the recipient strains. The PFK enzymes expressed appear to be stable in yeast. The human muscle...
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