Article
A single point mutation leads to an instability of the hetero-octameric structure of yeast phosphofructokinase.
The Biochemical journal - 1 Jul 1999
Kirchberger J, Edelmann A, Kopperschläger G, Heinisch J J
Abstract excerpt
Yeast phosphofructokinase is an oligomeric enzyme whose detectable activity in vitro depends on its hetero-octameric structure. Here we provide data demonstrating that an alanine residue at positions 874 (for the PFK1-encoded alpha-subunit) or 868 (for the PFK2-encoded beta-subunit) is crucial to achieve this structure. Thus subunits carrying substitutions by either aspartate or lysine of this residue cause a...
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