Article
Acetyl-CoA-dependent pyruvate carboxylase from the photosynthetic bacterium Rhodobacter capsulatus: rapid and efficient purification using dye-ligand affinity chromatography.
Microbiology (Reading, England) - 1 Oct 1995
Modak H V, Kelly D J
Abstract excerpt
Pyruvate carboxylase (PC) was purified to homogeneity from an overexpressing strain of the purple photosynthetic bacterium Rhodobacter capsulatus using a rapid dye-ligand affinity chromatography procedure, in which dye-bound enzyme was specifically eluted with a low concentration of acetyl-CoA, an allosteric activator of the enzyme. The enzyme purified by this method was obtained in 75% yield with a specific...
Topics
- Acetyl Coenzyme A
- Adenosine Diphosphate
- Allosteric Regulation
- Amino Acid Sequence
- Amino Acids
- Aspartic Acid
- Avidin
- Carbon Dioxide
- Chromatography, Affinity
- Coloring Agents
- Enzyme Activation