Article
Domain architecture of pyruvate carboxylase, a biotin-dependent multifunctional enzyme.
Science (New York, N.Y.) - 24 Aug 2007
St Maurice Martin, Reinhardt Laurie, Surinya Kathy H, Attwood Paul V, Wallace John C, Cleland W Wallace, Rayment Ivan
Abstract excerpt
Biotin-dependent multifunctional enzymes carry out metabolically important carboxyl group transfer reactions and are potential targets for the treatment of obesity and type 2 diabetes. These enzymes use a tethered biotin cofactor to carry an activated carboxyl group between distantly spaced active sites. The mechanism of this transfer has remained poorly understood. Here we report the complete structure of...
Topics
- Adenosine Triphosphate
- Allosteric Regulation
- Binding Sites
- Biotin
- Catalytic Domain
- Coenzyme A
- Crystallography, X-Ray
- Dimerization
- Enzyme Activators
- Models, Molecular
- Mutation