Modification of rhodamine staining allows identification of hematopoietic stem cells with preferential short-term or long-term bone marrow-repopulating ability.

doi:10.1073/pnas.92.19.8901

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Article

Modification of rhodamine staining allows identification of hematopoietic stem cells with preferential short-term or long-term bone marrow-repopulating ability.

Proceedings of the National Academy of Sciences of the United States of America - 12 Sept 1995

Zijlmans J M, Visser J W, Kleiverda K, Kluin P M, Willemze R, Fibbe W E

Abstract excerpt

We have developed a modified rhodamine (Rho) staining procedure to study uptake and efflux in murine hematopoietic stem cells. Distinct populations of Rho++ (bright), Rho+ (dull), and Rho- (negative) cells could be discriminated. Sorted Rho- cells were subjected to a second Rho staining procedure with the P-glycoprotein blocking agent verapamil (VP). Most cells became Rho positive [Rho-/Rho(VP)+ cells] and some...

Topics

Animals
Bone Marrow
Bone Marrow Cells
Bone Marrow Transplantation
Cell Separation
Female
Genetic Variation
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
In Situ Hybridization, Fluorescence

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

In Vitro Techniques

Mice

Mice, Inbred BALB C

Radiosurgery

Rhodamines

Spleen

Staining and Labeling

Survival Analysis

Source

Open in PubMed Open full text

Identifiers

PMID: 7568040
DOI: 10.1073/pnas.92.19.8901
PMCID: PMC41075

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7568040-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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