Adaptation of C6 glioma cells to serum-free conditions leads to the expression of a mixed astrocyte-oligodendrocyte phenotype and increased production of neurite-promoting activity.

Abstract excerpt

C6 cells were adapted to proliferate in defined culture medium to allow the study of long-term effects of serum-free growth conditions on their phenotypic antigen expression and production of neurite promoting factors (NPFs). Cultures were grown in either Ham's F-12 or supplemented Opti-MEM-I containing 15% heat-inactivated horse serum and 2.5% fetal calf serum (serum-containing) or in supplemented Opti-MEM-I...

Topics

• Astrocytes
• Cell Division
• Culture Media, Serum-Free
• Fluorescent Antibody Technique
• Gene Expression
• Glioma
• Immunohistochemistry
• Neurites
• Oligodendroglia
• Phenotype
• Tumor Cells, Cultured