Purification and characterization of wild-type and ts 112 mutant protein IIIa of human adenovirus 2 expressed in Escherichia coli.

doi:10.1016/0042-6822(90)90202-3

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Article

Purification and characterization of wild-type and ts 112 mutant protein IIIa of human adenovirus 2 expressed in Escherichia coli.

Virology - 1 Mar 1990

Cuillel M, Cortolezzis B, Chroboczek J, Langowski J, Ruigrok R W, Jacrot B

Abstract excerpt

The expression of the protein IIIa gene from human adenovirus type 2 (Ad2) in Escherichia coli has been described previously (M. Cuillel, M. Milleville, and J. C. D'Halluin, 1987, Gene 55, 295-301). The same construct has now been used to express a protein IIIa gene from an Ad2 mutant ts 112 whos...

Topics

Adenoviruses, Human
Capsid Proteins
Centrifugation, Density Gradient
Cloning, Molecular
Electrophoresis, Disc
Escherichia coli
Genetic Vectors
Immune Sera
Immunoblotting
Light
Microscopy, Electron
Mutation
Protein Conformation
Recombinant Proteins
Restriction Mapping
Scattering, Radiation
Viral Proteins

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PMID: 2408227
DOI: 10.1016/0042-6822(90)90202-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2408227-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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